Nucleic acids are important chemical compounds that can be found everywhere around us. They play a critical role in laboratory testing for early detection of diseases in humans and the biosphere surrounding us. Nucleic acid extraction can be the groundsel of many genetic and genomic studies. Acid extractions requires laboratory equipment, expertise and proper equipment.

Standard laboratory extraction procedure involves three major steps: lysis of cell nucleus membranes with surfactants (e.g., SDS, CTAB, or Triton X-100), denaturation of proteins by proteases (e.g., proteinase K), and purification of nucleic acids [1] [3]. However, the actual extraction protocols vary significantly depending on the purposes of sample preparation. For example, for genomic DNA isolation, white blood cells need to be separated from the rest of the blood components [2]. In contrast, for the detection of pathogenic nucleic acids or cell-free DNAs, either serum or pathogen-infected blood cells are separated before extraction [7]. Removing red blood cells also helps to reduce the inference of hemoglobin, which is one of the major sources of inhibitors for downstream NAA reactions [4] [5].

For DNA isolation magnetic beads (MBs) are used as a tool for nucleic acid extraction. Coupling of magnetic properties with specific ligands in MBs allows the separation and purification of nucleic acids in a highly efficient and specific manner. In fact, this technique has driven a technological revolution in biological research. [6]